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. 2023 Aug 4;13:12687. doi: 10.1038/s41598-023-39555-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Multiple cecal patches are present in Nlgn3^R451C mice.Representative images of pinned-out cecum from WT (A) and Nlgn3 (B) mice. Blue dashes outline the main cecal patch, whereas orange dashes outline the isolated lymphoid follicles (ILF) typically found along the body of the cecum. (C) Total cecal patch numbers are significantly higher in Nlgn3 cecum compared with WT littermates. 3 outliers were removed in the WT group according to the ROUT Q = 1% method. (D) When total cecal patch count is split into main and ILF cecal patches, the difference is not significant despite an evident, qualitative trend for an increase in Nlgn3 ILF. Statistical comparisons were conducted using Student’s unpaired t-test and individual data and mean ± SD were plotted for WT: n = 12; Nlgn3: n = 16 mice in each group. ns = p > 0.05, *p < 0.05. WT = wildtype; Nlgn3 = Nlgn3^R451C.