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. 2023 Aug 4;13:12687. doi: 10.1038/s41598-023-39555-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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No morphological difference was observed in Iba-1⁺ immunopositive cells in the myenteric plexus of Nlgn3^R451C cecum compared to WT littermates. 3D reconstruction and analysis of Iba-1⁺ cells in WT (A) and Nlgn3 (B) myenteric plexus. No significant morphological differences were observed with the parametrized batches of Iba-1⁺ cells for (C), area (D), sphericity (E), and volume (F). Statistical comparisons were conducted using Student’s unpaired t-test. Individual data and mean ± SD were plotted for n = 5 mice in each group. ns = p > 0.05. WT = wildtype; Nlgn3 = Nlgn3^R451C.