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. 2023 Aug 4;13:12674. doi: 10.1038/s41598-023-39527-2

Table 2.

Results of behavioural experiments using the Y-tube olfactometer on adult Zenopontonia soror.

Aquarium A VS aquarium B Results Statistics
Trials Null A B Motion behaviour Orientation behaviour
Negative control
Seawater (A) VS seawater (B) 20 18 1 1 1
Water conditioned by the initial host
Culcita novaeguineae (A) VS seawater (B) 40 4 33 3 1.8 × 1010 2.3 × 107
Water conditioned by asterosaponins extracted from potential host asteroid species
C. novaeguineae extract (A) VS seawater (B) 40 4 33 3 1.8 × 1010 2.3 × 107
Acanthaster planci extract (A) VS seawater (B) 20 13 6 1 0.1 0.1
Linckia laevigata extract (A) VS seawater (B) 20 15 5 0 0.4 6 × 102
Water conditioned by saponins from non-host echinoderm species
Asterias rubens extract (A) VS seawater (B) 20 18 1 1 1 1
Echinaster sepositus extract (A) VS seawater (B) 20 18 1 1 1 1
Holothuria scabra extract (A) VS seawater (B) 20 18 1 1 1 1

Eight different tests were performed on a minimum of 20 different adult shrimps. Results show the number of trials, spread out among the shrimps that remained stationary in the beginning of the impaired branch (Null), those that orientated adequately towards the cues source (A) and those of orientated towards the control aquarium (B). The P values of the chi2 test and the binomial test are respectively found in motion and orientation behaviour columns. Significant results (P value, 0.01) are underlined and bolded.