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. 2023 Aug 4;13:12674. doi: 10.1038/s41598-023-39527-2

Table 3.

Results of behavioural experiments using the olfactometer on larvae of Zenopontonia soror.

Aquarium A VS aquarium B Results Statistics
Trials Null A B Motion behaviour Orientation behaviour
Negative control
Seawater (A) VS seawater (B) 20 12 4 4 1
Water conditioned by the initial host
Culcita novaeguineae (A) VS seawater (B) 40 4 33 3 1.3 × 10–4 2.3 × 107
Water conditioned by asterosaponins extracted from potential host asteroids
C. novaeguineae extract (A) VS seawater (B) 40 5 33 2 3.9 × 104 3.7 × 108
Acanthaster planci extract (A) VS seawater (B) 40 6 32 2 1 × 103 6.4 × 108
Comparison of two waters conditioned by asteroids saponins extracts
C. novaeguineae extract (A) VS A. planci extract (B) 40 8 29 3 4.5 × 103 2.6 × 105

Five different tests were performed on a minimum of 20 different larvae. Three individuals of Culcita novaeguineae were used to prepare conditioned water and, for each saponins extract, at least 3 individuals for each species were used. Results show the number of trials, spread out among the shrimps that did not converge significatively toward the device (Null), those that orientated adequately toward the cues from aquarium A (A) and those that orientated toward the cues coming from the aquarium B (B). The P value of the chi2 test and the binomial test are respectively found in motion and orientation behaviour columns. Significant results (P value, 0.01) are underlined and in bold.