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. 2023 Aug 4;14(8):500. doi: 10.1038/s41419-023-05995-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Confocal micrographs of the staining for GFAP (red) and Kidins220 (green) in the SEZ of wild-type, Kidins220^f/f and Kidins220^GfapΔ/Δ mice. Nuclei are stained with DAPI (blue). Scale, 50 μm, and 10 μm (insets). LV, lateral ventricle (delimited by white dashed lines). White arrowheads point at GFAP positive cells. B Representative Kidins220 and ß-actin (loading control) immunoblots of SEZ lysates from wild-type, Kidins220^f/f and Kidins220^GfapΔ/Δ mice. Each lane represents extracts from one mouse. C, Kidins220 levels represented in arbitrary units after normalization with ß-actin in wild-type, Kidins220^f/f and Kidins220^GfapΔ/Δ mice. Data represent mean ± s.e.m.; each data point represents an individual mouse (N = 3, for each condition). *P < 0.05, **P < 0.01, ***P < 0.001, by one-way ANOVA followed by Tukey’s post-hoc test. D Confocal micrographs of the staining for GFAP (red) and Kidins220 (green) in the DG of wild-type, Kidins220^f/f and Kidins220^GfapΔ/Δ mice. Nuclei are stained with DAPI (blue). Scale bars: 25 μm, and 10 μm (insets). SGZ, subgranular zone: GL, granule cell layer; White arrowheads point at GFAP⁺ cells (white dashed lines). E Representative Kidins220 and ß-actin (loading control) immunoblots of hippocampal lysates from wild-type, Kidins220^f/f and Kidins220^GfapΔ/Δ mice. Each lane represents extracts from one mouse. F Kidins220 levels represented in arbitrary units after normalization with ß-actin in hippocampal lysates in wild-type, Kidins220^f/f and Kidins220^GfapΔ/Δ mice (N = 3, each). Data represent mean ± s.e.m.; each data point represents an individual mouse. *P < 0.05, **P < 0.01, ***P < 0.001, by one-way ANOVA followed by Tukey’s post-hoc test.