Fig. 7.

The fluoride-induced key gut microbiota could colonize in pseudo-germfree mice by FMT. Gut microbiota was analyzed by 16S rRNA gene sequencing (n = 8 for each group). (A) PCA based on the relative abundance of Features of the gut microbiota, (B) PCoA of the gut microbiota based on the unweighted unifrac distance matrix, (C) Bacterial taxonomic profiling at the phylum level of gut microbiota, the relative abundances of (D) Firmicutes, (E) Bacteroidetes, and (F) the ratio of Firmicutes to Bacteroidetes. (G-H) LEfSe analyses of gut microbiota (value of Kruskal-Wallis: 0.05, value of Wilcoxon: 0.05, value of LDA score: 3.5). the relative abundances of (I) Erysipelotrichaceae, and (J)Erysipelatoclostridium. The results were expressed as means ± SEM. Difference in two groups was calculated using the Mann-Whitney test or Kruskal-Wallis test. A value of p < 0.05 was considered to be significant.