Fig. 10.

E. ramosum aggravates the obesity in HFD mice. (A) Scheme of animal experiment 8 over the 8 weeks. Pseudo-germfree mice were obtained by treatment of Abx for two weeks. Then, pseudo-germfree mice were randomly divided into two groups including HFD fed with PBS and HFD fed with E. ramosum (HFD-ER) by intragastric gavage for two weeks. (B) Dynamic changes in body weight in mice. (C) Body weight gain. (D) Epididymal fat. (E) Mesentery fat. (F) Perirenal fat. (G-H) Representative morphology and H&E staining of Epididymal fat. (I) Adipocytes (μm²) distribution in the Epididymal fat tissue. (J-K) Plasma levels of TC and LDL-C. (L) OGTT was carried out at week 7, mice were fasted overnight and gavaged with a dosage of glucose with 1.5 mg/g body weight (n = 5 per group). (M) AUC for OGTT. (N) Liver weight. (O) Liver TG. (P) Plasma ALT. (Q-S) Representative morphology, H&E staining and Sirius Red histology of liver. The results were expressed as means ± SEM. Difference in two groups was calculated using the Mann-Whitney test or Kruskal-Wallis test. A value of p < 0.05 was considered to be significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)