FIG. 2.

Accumulation of an IE protein (ICP22) and an L protein (VP16) in infected nonhuman (A and B) and human (C to E) cell lines. Total cell extracts (50 μg) prepared at 24 h p.i. from mock- (M), vBSΔ27 (Δ27)-, vBSΔ27R (Δ27R)-, and KOS1.1-infected nonhuman Vero, Vero 2.2, and human 143tk⁻, HEp-2, and HeLa cells were used for immunoblot analyses with the polyclonal anti-ICP22 antibody RGST22 and the monoclonal anti-VP16 antibody as described in Materials and Methods. The bars indicate the multiple electrophoretic forms of ICP22.