Skip to main content
. Author manuscript; available in PMC: 2024 Mar 24.
Published in final edited form as: J Vis Exp. 2023 Mar 24;(193):10.3791/65083. doi: 10.3791/65083

Figure 1: Schematic for the lentiviral plasmid expressing Cas9 and sgRNA.

Figure 1:

Lentiviral expression vector for Cas9 and sgRNA (lentiCRISPRv2). Abbreviations: Puro = puromycin selection marker; psi+ = psi packaging signal; RRE = rev response element; cPPT = central polypurine tract; EFS = elongation factor-1α short promoter; P2A = 2A self-cleaving peptide; WPRE = posttranscriptional regulatory element; LTR = long terminal repeat. LentiCRIPSRv2 can be digested using BsmBI, and a pair of annealed sgRNA oligos can be cloned into the single-guide RNA scaffold.