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. Author manuscript; available in PMC: 2024 Mar 24.
Published in final edited form as: J Vis Exp. 2023 Mar 24;(193):10.3791/65083. doi: 10.3791/65083

Figure 2: Schematic for the AAV plasmid expressing sgRNA.

Figure 2:

The AAV vector for sgRNA. Abbreviations: ITR = I terminal repeat; CB = hybrid CMV enhancer/β-actin promoter. pAAV-U6-BbsI-gRNA-CB-EmGFP can be digested using BbsI, and a pair of annealed sgRNA oligos can be cloned into the single-guide RNA scaffold.