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. Author manuscript; available in PMC: 2024 Mar 24.
Published in final edited form as: J Vis Exp. 2023 Mar 24;(193):10.3791/65083. doi: 10.3791/65083

Table 1: Example of sgRNA design.

Example of an sgRNA sequence with overhangs corresponding to the BsmBI restriction site.

Forward oligo: 5’ CACC-20bp gRNA sequence-3’
Reverse oligo: 5’ AAAC-20bp gRNA reverse-complement sequence-3’
For example, if the target sequence is TCTGATAGCGTAGGAGTGAT, the oligos would be:
Forward oligo: 5’ CACC TCTGATAGCGTAGGAGTGAT 3’
Reverse oligo: 5’ AAAC ATCACTCCTACGCTATCAGA 3’