List of Material and equipment
| Name of Material/ Equipment | Company | Catalog Number |
|---|---|---|
| Chemicals, Peptides and Recombinant Proteins | ||
| TransIT®-LT1 Transfection Reagent | Mirus | MIR 2300 |
| Polybrene Infection / Transfection Reagent | Sigma | TR-1003-G |
| OPTI-MEM serum-free medium | Invitrogen | 31985 |
| Recombinant DNA | ||
| lentiCRISPR v2 vector | Addgene | 52961 |
| pAAV-U6-BbsI-gRNA-CB-EmGFP | Addgene | 89060 |
| psPAX2 packaging plasmid | Addgene | 12260 |
| pMD2.G envelope plasmid | Addgene | 12259 |
| Experimental Models: Cell Lines | ||
| HEK-293 cells | ATCC | CRL-1573 |
| WT-1 cells | Developed in Tseng lab | PMID: 14966273 |
| Experimental Models: Organisms/Strains | ||
| Cas9 knockin mice | Jackson Laboratories | 24857 |
| Ucp1-CRE mice | Jackson Laboratories | 24670 |
| Others | ||
| Banamine | Patterson | 07-859-1323 |
| Hamilton syringe | Hamilton | Model 710 RN Syringe |
| Needle | Hamilton | 32 gauge, small hub RN needle, needle point style 2 |
| Suture 5-0 Coated Vicryl Undyed 1X27” | Ethicon | J421 |
| Suture 5-0 Unify PCL25 P-3 Undyed 18” Monofilament 12/Bx | Henry Schein | 1273504 |
| hydrogen peroxide solution | Fisher Scientific | H325-500 |