Table 3.
List of the designed primers and their Tms, IDs, sequences, the amplicon ID and length
| Primers ID | Tm oC | primer sequence 5’ > 3’ | Amplicon ID according to database | Amplicon length/ bp | ||
|---|---|---|---|---|---|---|
| 27 F | 50 | AGAGTTTGATCCTGGCTCAG | 16 S rDNA gene | 1500 | ||
| 1492R | GGTTACCTTGTTACGACTT | |||||
| NarX-F | 55 | GGATGAAATGGCGTCGCTTG | nitrate/nitrite two-component system sensor histidine kinase, NarX | 268 | ||
| NarX-R | TTCCCGGCTGTCGTTTTCAT | |||||
| NarQ-F | CTGTTCGGCATCGTGATACT | nitrate/nitrite two-component system sensor histidine kinase, NarQ | 266 | |||
| NarQ-R | TGCAGCGCCAGATAGTGGTT | |||||
| NarK-F | CCGTAGAAGGCGATGAACGA | NarK family nitrate/nitrite MFS transporter | 212 | |||
| NarK-R | ACGCAATTTCCGGAGGTCGT | |||||
| oxyred-F | CAGATCGTTCAGGCCCATCG | Putative Oxidoreductase, UbiI “SMDB11_RS16455” | 256 | |||
| oxyred-R | CGAGCTGATTTCCGAACTGC | |||||
| NTR-F | ATGAATTGCTGCACCGGCTC | Putative nitroreductase “SMDB11_RS08680” | 259 | |||
| NTR-R | ACGTGATGACGCTCGAAGAG | |||||
| NapC/NirT-F | AACCTGCATCGATTGCCACA | NapC/NirT family cytochrome c (nitrate reductase) | 378 | |||
| NapC/NirT-R | GTTTCCGCATCGGTCTGAGTC | |||||
| AmoA-1 F | GGGGTTTCTACTGGTGGT | alpha subunit of ammonia monooxygenase gene | - | |||
| AmoA-1R | CCCCTCKGSAAAGCCTTCTTC [K = G or T; S = G or C] | |||||
| NxrA- F | CAGACCGACGTGTGCGAAAG | nitrite oxidase, NxrA | - | |||
| NxrA- R | TCCACAAGGAACGGAAGGTC | |||||