Table 2.

Amplification conditions for interleukin-10 (rs1800872 and rs1800896) polymorphisms

Serial number	Steps	rs1800872		rs1800896
		Temperature (°C)	Time	Temperature (°C)	Time
1	Initial denaturation	95	5 min	95	1 min
2a	Denaturation	95	45 s	95	15 s
b	Annealing	62	30 s	65	50 s
c	Extension	72	45 s	72	25 s
		Steps 2a–2c repeated for 5 cycles		Steps 2a–2c repeated for 10 cycles
3a	Denaturation	95	45 s	95	15 s
b	Annealing	61	50 s	61	50 s
c	Extension	72	45 s	72	45 s
		Steps 3a–3c repeated for 5 cycles		Steps 3a–3c repeated for 10 cycles
4a	Denaturation	95	30 s	95	30 s
b	Annealing	59	45 s	61	50 s
c	Extension	72	45 s	72	50 s
		Steps 4a–4c repeated for 30 cycles		Steps 4a–4c repeated for 20 cycles
5	Final extension	72	10 min	72	10 min
6	Hold	4	Infinity	4	Infinity