TABLE 3.
Infection of various cell types with HIV-GFP/SIVpack/G and LNCX-GFP/Ψ(−)env(−)ampho/Ga
| Target cells | % of GFP-positive cells after exposure to:
|
|
|---|---|---|
| HIV-GFP/ SIVpack/G | LNCX-GFP/Ψ(−) env(−)ampho/G | |
| CEMX174 | 11 | ND |
| Primary human macrophages | 15 | <0.1 |
| Primary mouse neurons | 15 | <0.1 |
The indicated cells were exposed to HIV-GFP/SIVpack/G and LNCX-GFP/Ψ(−)env(−)ampho/G at an MOI of 1.0, chosen based on vector titrations on HeLa cells. Except for mouse neurons, cells were infected in the presence of Polybrene at 10 μg/ml. At 48 or 72 h postinfection, fluorescent cells were visually counted under a microscope. Mock infections of the same cell types were performed in parallel and resulted in no detectable fluorescent cells. ND, not determined.