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. 2023 Aug 5;13:12720. doi: 10.1038/s41598-023-37874-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PP2A-B55alpha regulates epithelial cell adhesion and barrier through DP. (A) A dispase-adhesion assay was performed in SCC9s transfected with siRNA targeting Scramble, B55⍺, or DP and grown in normal HCM for 5 days. Images represent SCC9 monolayers pre- (left) and post- (right) exposure to mechanical stress. (B) Monolayer fragmentation from (A) was quantified and represented as total fragment number from n = 5. (C) Western blot validation of siRNA knockdown in (A). (D) A dispase-adhesion assay was performed in A431 cells expressing either dox-inducible DPserGFP or DPglyGFP and transfected with siRNA targeting Scramble, B55⍺, or DP. Cells were grown in normal HCM with 1 μg/mL Dox for 6 days. Images represent A431 monolayers pre- (left) and post- (right) exposure to mechanical stress. (E) Monolayer fragmentation from (A) was quantified and represented as total fragment number from n = 4. (F) Western blot validation of siRNA knockdown in (C). Statistical analyses were performed using a One-way ANOVA with multiple comparisons. * < 0.05; ** < .01.