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. 2023 Aug 5;14:4706. doi: 10.1038/s41467-023-40419-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Representative 3D dSTORM reconstruction of LRRTM2 (cyan) and Homer1 (red). b LRRTM2 Homer1 synaptic abundance n = 232 synapses from 3 independent experiments (left) and number of SSDs n = 151 synapses from 3 independent experiments (right). LRRTM2 localization distance (c) and SSD distance (d) to Homer1 centroid. The mean and 95% confidence interval of Poisson fitted distribution are indicated on the graph. e Representative 3D dSTORM reconstruction of LRRTM2 (cyan) and Nrxn1 (left) or Nrxn3 (right; red) at Homer1+ synapse (green) in an enface view. f Nrxn1 is farther from LRRTM2 SSDs than Nrxn3, p = 0.0135; Mann–Whitney test (two-tailed). g Representative 3D dSTORM reconstruction of GluD1 (cyan) and Homer1 (red) in an enface view. h GluD1 Homer1 synaptic abundance n = 196 synapses from 3 independent experiments (left) and number of SSDs n = 134 synapses from 3 independent experiments (right). i–j GluD1 localization distance (g) and GluD1 SSD distance (h) from the Homer1 centroid. The mean and 95% confidence interval of the Poisson fitted distribution indicated on the graph. k Representative 3D dSTORM reconstruction of GluD1 (cyan) and Nrxn1 (left) or Nrxn3 (right; red) at Homer1+ synapse (green) in an enface view. l Nrxn1 is closer to GluD1 SSDs than Nrxn1, p = 0.0314; Mann–Whitney test (two-tailed). Number of synapses and independent experiments are indicated in the figure. Bar graphs and line graphs: average ± SEM. Violin plots: median ± upper and lower quartiles. *p < 0.05. Source data are provided as a Source Data file.