Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 5;14:4716. doi: 10.1038/s41467-023-40203-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — A Schematic showing analysis timeline for cardiomyocyte specific Arid1a mutants (Arid1a cKO). B Representative images of hematoxylin and eosin (H&E) staining of P7 and P14 control and Arid1a cKO hearts. C Uneven surface (arrowheads) and signs of myocardial disarray (bottom) in Arid1a cKO hearts (n = 4) compared to controls (n = 5) at P14. D Heart weight to body weight ratio (HW/BW) at P7 (Control, n = 9; Arid1a cKO, n = 4) and P14 (Control, n = 3; Arid1a cKO, n = 4; P = 0.0029; **P < 0.01, two-way ANOVA with Šídák’s multiple comparisons test). E Sirius red (SR) staining for fibrosis and wheat germ agglutinin (WGA) staining for extracellular matrix. F Quantification of cardiomyocyte cross-sectional area (CSA) as measured from WGA-stained hearts at P7 (n = 8) and P14 (Control, n = 3; Arid1a cKO, n = 4) revealed no significant difference between Arid1a cKO and control at P7 (P = 0.2049) or P14 (P = 0.1021; two-way ANOVA with Šídák’s multiple comparisons test). G Representative example of echocardiography analysis of cardiac function in P7 hearts, with quantification of fractional shortening (P < 0.0001), interventricular septum thickness (IVS; P = 0.7787) and left ventricular peripheral wall thickness (LVPW; P = 0.3624) during diastole (d) (Control, n = 12; Arid1a cKO, n = 9; ****P < 0.0001, two-tailed Student’s t-test). Scale bars 1 mm (B), 25 µm (C), 50 µm (E), 0,1 s (G, horizontal), 0,1 mm (G, vertical). All bar graphs show mean with standard error of the mean. Source data are provided as a Source Data file.