FIG. 12.

Western blot analysis of immunoprecipitates reveals that IEV proteins interact with each other but not with the IMV-associated proteins A27L and D8L. Immunoprecipitations were performed on cell extracts prepared at 24 h postinfection with either control immunoglobulin G (IgG) or antibodies against A36R and B5R as indicated at the top. S and P, supernatant and pellet from the immunoprecipitation, respectively; E, untreated control extract. Western blots were probed with antibodies against A36R, A33R, A34R, B5R, A27L, and D8L as indicated on the left. Identical results were obtained with cell extracts prepared at 8 h postinfection (data not shown).