FIG. 13.

Western blot analysis of immunoprecipitates with extracts prepared from ΔF13L-, ΔA34R-, or ΔA36R-infected cells. Immunoprecipitations were performed with antibodies against A36R (left panel) or B5R (right panel). S and P, supernatant and pellet, respectively; E, untreated extract prepared from WR-infected cells. Western blots were probed with antibodies against A33R (left panel) or A34R (right panel), showing that the interaction of A36R with A33R as well as the interaction of B5R with A34R is not dependent on IEV formation as they are obtained in extracts from ΔF13L-infected cells. Both interactions are also preserved in the absence of A34R and A36R, respectively, as demonstrated with extracts from ΔA34R- and ΔA36R-infected cells.