FIG. 2.
Effect of point mutations around the sgRNA1 start site. Northern blot analysis shows gRNA and sgRNA1 accumulation in protoplasts inoculated with mutant full-length genomic transcripts. Total RNA from oat protoplasts (24 hpi) was hybridized with a 32P-labeled transcript complementary to bases 2737 to 2985 of BYDV gRNA. Each mutant was analyzed in duplicate. Altered nucleotides and amino acids are in boldface. Arrows above each sequence show the sgRNA1 transcription start site identified by Kelly et al. (18) (position 2670).