Skip to main content
. 2023 Jun 9;16(3):451–472. doi: 10.1016/j.jcmgh.2023.06.003

Figure 1.

Figure 1

Generation of isogenic iPSC lines. (A) Sanger DNA sequencing tracks showing correction of the DKC1 mutation in patient-derived iPSCs. The PAM sequence for the RNA was mutated by using a GCT codon at A353 instead of the wild-type codon (GCG) for the correction. (B) Immunostaining of pluripotency markers Nanog and Sox2 in the isogenic iPSC pair. Scale bar: 100 μm. (C) Karyotyping of metaphase iPSCs. (D) Telomerase activity in iPSCs measured by qTRAP assay (n = 3). ∗∗P < .01. Error bars indicate means ± SD. (E) Telomere length in iPSCs measured by TeSLA. (F) Teratoma formation from iPSCs showing ectodermal derivatives (neuroepithelium), mesodermal derivatives (cartilaginous condensations), and endodermal derivatives (glandular/secretory epithelium) in both corrected and mutant tumors. Scale bar: 50 μm. Cor, corrected; Mut, mutant.