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. 2023 Jun 9;16(3):451–472. doi: 10.1016/j.jcmgh.2023.06.003

Figure 10.

Figure 10

Pharmacological rescue of DC phenotypes in hepatostellate organoids. (A) Schematic of drug treatment period during organoid induction. (B) Quantification of organoid diameter in response to indicated drug treatment (n = 20). Error bars indicate mean ± SD. (C) Whole-mount staining of Cleaved Caspase-3 and HNF4α in hepatostellate organoids. Scale bar: 200 μm. (D) BODIPY staining of hepatostellate organoids. Scale bar: 100 μm. (D) Gene expression analysis and immunofluorescence staining in DKC1 ΔL37 mutant HEPs treated with AKT inhibitor. (n = 4). Error bars indicate mean ± SD. Scale bar = 100 μm. (E) Quantification of DKC1 ΔL37 pair organoid diameter in response to AKT inhibitor (n = 30). Error bars indicate mean ± SD. (G) Representative images showing Telomere Dysfunction Induced Foci (TIFs), quantified at right (n = 3). Scale bar: 10 μm. (H) Representative images showing 53BP1 and γ-H2A.X staining in HEP cultures. Scale bar: 100 μm. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗∗P < 0.0001.