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. 2023 Jun 9;16(3):451–472. doi: 10.1016/j.jcmgh.2023.06.003

Figure 8.

Figure 8

Endothelial cells associated with DKC1-mutant hepatocytes exhibit proinflammatory phenotypes. (A) Cell composition distribution within hepatostellate organoids. (B) Expression histograms of PLVAP and Notch ligands (JAG1, JAG2, and DLL4) in indicated cell cluster from hepatostellate organoid cultures. (C) Violin plot showing Notch receptor expression across hepatostellate organoid genotypes. (D) Gene expression heatmap visualizing highly expressed genes from the endothelial cell cluster in bulk 2D HB and HEP cultures. (E) Staining of HNF4α and CD34 in 2D HEP cultures (scale bar = 100 μm); CD34-positive cells were quantified at right (n = 3). (F) Immunofluorescence images of HNF4α and Ki67 in HEP cultures of indicated genotypes treated with the γ-secretase inhibitor dibenzazapine (DBZ, (10 μmol/L). Scale bar: 100 μm. Graphs show quantification of images (n = 3). (G) Representative morphology of hepatostellate organoids and quantification of organoid diameter (n = 20). Scale bar: 100 μm. ∗∗P < .01 ∗∗∗∗P < .0001. Error bars indicate means ± SD. Cor, corrected; Mut, mutant.