Figure 2.

oxLDL uptake induces foam cell formation in M0 and M2c macrophages. (A,B) oxLDL uptake in macrophages was determined based on ORO staining. Cells were observed under light microscopy (40× objective lens), and ORO-stained areas were analyzed. Stained areas in M0 and M2c macrophages were normalized to ORO-stained cell number (A), and representative images are shown (B). Bars represent mean ± SEM from five independent experiments. (C) Alexa Fluor 647-conjugated oxLDL uptake in M0 and M2c macrophages was analyzed by flow cytometry, and representative histograms from three independent experiments are shown. Dotted and solid lines: oxLDL treatments at 0 and 50 µg/mL, respectively.