Figure 3.

Lipid uptake and cholesterol efflux activity in lysoPtdGlc-stimulated M2c macrophages. (A) M2c macrophages were treated with ML193 (GPR55 antagonist) for 2 h, stimulated with 10 nM lysoPtdGlc for 2 h, incubated with 50 µg/mL oxLDL for 24 h, stained with ORO, and dissolved in isopropanol. Absorbance of supernatants was measured at wavelength 490 nm. (B,C) ABCA1-mediated (B) and ABCG1-mediated (C) cholesterol efflux in M2c macrophages was analyzed using TopFluor cholesterol under various conditions as indicated. Cells were treated sequentially with ML193, lysoPtdGlc, and oxLDL as in A. Data were normalized to non-treated cells. Bars represent mean ± SEM from three independent experiments. Data analysis and notations as in Fig. 1.