TABLE 2.
Expression of a transgene containing an intron is enhanced by the WPRE
| Vector | Luciferase expression in the following transduced cellsa:
|
||
|---|---|---|---|
| HeLa | HeLa-tat | 293T | |
| HR′Luc | 10,840 ± 59 | 359,931 ± 3,972 | 66,936 ± 1,842 |
| HR′Luc-W | 48,835 ± 491 | 1,904,711 ± 60,525 | 588,755 ± 30,544 |
| WPRE/control ratio | 4.2 | 5.3 | 8.8 |
a
Reported as relative light units per nanogram of p24 (mean ± standard error of the mean). For each vector type, 50 μl of two independently produced stocks representing 0.5 to 1 ng of p24 was used to transduce in parallel and in duplicate 2 × 105 cells. The estimated MOI was <0.01.