Table 1.

Summary of isothermal titration calorimetry (ITC) cocaine binding data for control and FITC labeled h2E2 mAb.

Sample	Average mol FITC/mol mAb	N (cocaine sites/Fab)	K (M−1)	ΔH (cal/mol)	ΔS (cal/mol/deg)	ΔG (cal/mol)	Kd (nM)
control h2E2 mAb	0	0.782	1.60E+08	−13800	−9.57	−11000	6.25
15 min FITC-h2E2 mAb	1.91	0.706	1.11E+08	−14400	−12.5	−10800	9.01
30 min FITC-h2E2 mAb	2.88	0.789	1.15E+08	−12900	−7.26	−10800	8.70
60 min FITC-h2E2 mAb	3.83	0.707	7.58E+08	−13200	−8.98	−10600	13.2
90 min FITC-h2E2 mAb	4.46	0.748	8.45E+07	−12700	−6.91	−10600	11.8
120 min FITC-h2E2 mAb	5.00	0.738	8.23E+07	−12400	−6.01	−10600	12.2