Fig. 2.

Ca²⁺dynamics. a. Representative fluorescent Ca²⁺ time-lapse video recordings of null and CHRFAM7A KI isogenic iPSC derived MGE progenitors. b. Representative fluorescent Ca²⁺ traces from cells marked in videos in a. c. Representative time-lapse video frames demonstrating the Ca²⁺ signal change over time in null and CHRFAM7A MGE progenitors (white box active Ca²⁺ signal time interval in Null, red box active Ca²⁺ signal time interval in CHRFAM7A KI MGE progenitors). d. Cumulative density curves of Ca²⁺ peak characteristics in null and CHRFAM7A KI isogenic iPSC derived MGE progenitors. Operator independent analysis of single cell Ca²⁺ tracings was performed in MatLab. (3 independent experiments, 50–100 peaks per cell line). Statistical comparison was performed using the two-sample Kolmogorov–Smirnov test. Multiple testing correction was performed by the Bonferroni method. (A amplitude, FWHM form width at half maximum amplitude). e. Proposed mechanism of CHRFAM7A modified Ca²⁺ Flux (created with BioRender.com). f. Ca²⁺ dynamics curve morphology indicates a shift in time occupancy of CICR to IICR in the presence of CHRFAM7A.