Fig. 5.

CHRFAM7A effect on structure of the synapse. a. Schematic depicting dendritogenesis timeline and formation of synapsis (created with BioRender.com). b. Representative Synaptophysin and MAP2 confocal images demonstrating a difference in dendritic spinogenesis in neurons (D 30 after plating MGE progenitors) derived from null and CHRFAM7A KI lines. Quantification of number (c.) and area (d.) of synapsis on neurons derived from both lines. Statistical comparison was performed using the two-sample Kolmogorov–Smirnov test. Representative immunoblot (e.) and densitometric analysis (f.) of presynaptic (synaptophysin) and postsynaptic (PSD95) markers in a crude synaptosome fraction isolated from the null and CHRFAM7A KI iPSC derived neurons (n = 3 independent experiments, independent two-tailed T-test). g. Representative dendritic spine images of null and CHRFAM7A KI iPSC derived neurons. The neurons are stained with phalloidin followed by confocal microscopy and ImageJ processing. h. Dendritic spine length difference between the null UB068 (grey) and CHRFAM7A (orange). (N = 50 from 3 independent experiments, independent two-tailed T-test). i. Schematic depicting dendritic spine types and colour code for quantification (created with BioRender.com). j Quantification of dendritic spines in neurons derived from two iPSC lines. 20 images from 3 independent experiments. Statistical analysis was performed by non-parametric two-tailed Mann–Whitney test.