Table 1.

Incorporation efficiency of Met analogs in nisin and its variants

Peptide	Analog	Incorporation efficiency
		pCZ-nisin	pNZ-nisin
Wild type	Aha	88%	>99.5%
	Nle	77%a	>99.5%
	Eth	56%a	>99.5%
M17IM21V-I1M	Aha	91%	>99.5%
	Nle	88%a	>99.5%
	Eth	71%a	>99.5%
M17I	Aha	97%	>99.5%
	Nle	88%a	>99.5%
	Eth	71%a	>99.5%
M21V	Aha	96%	>99.5%
	Nle	88%a	>99.5%
	Eth	73%a	>99.5%
M17IM21V-35M	Aha	>99.5%	>99.5%
	Nle	51%a	>99.5%
	Eth	71%a	>99.5%
nisin(1–21)M17I	Aha	n.d	>99.5%
	Nle	n.d	>99.5%
	Eth	n.d	>99.5%
nisin(1–21)M21V	Aha	n.d	>99.5%
	Nle	n.d	>99.5%
	Eth	n.d	>99.5%
nisin(1–22)M17I	Aha	n.d	>99.5%
	Nle	n.d	>99.5%
	Eth	n.d	>99.5%
nisin(1–22)M21V	Aha	n.d	>99.5%
	Nle	n.d	>99.5%
	Eth	n.d	>99.5%

>99.5% means the mass of the peptides containing Met was undetectable. n.d, not detected.

^aResults from reference (14).