Table 1.
Cryo-EM data collection, refinement, and validation statistics for Btbc1/CK-2-68 complex
| Data collection | |
| Grid type | Quantifoil R1.2/1.3400 mesh copper |
| Microscope | FEI Titan Krios |
| Detector | Gatan K2 Summit (4K x 4K) |
| Magnification | 58,275 |
| Voltage (kV) | 300 |
| Energy filter slit (eV) | None |
| Image mode | Super resolution |
| Electron dosage (e-/Å2) | 50.24 |
| Electron dose per frame (e-/Å2) | 1.0048 |
| Defocus range (μm) | −1.25 to −2.5 |
| Pixel size (Å) | 0.858/0.429 |
| Raw micrographs | 6960 |
| Data processing | |
| Extracted initial particles (no.) | 883,119 |
| Selected final particles (no.) | 454,182 |
| Particles for final map (no.) | 454,182 |
| Symmetry imposed | C1 |
| Map resolution (Å) | 2.88 |
| FSC threshold | 0.143 |
| Refinement | |
| Initial model used (PDB code) | 1SQX |
| Model resolution (Å) | 2.88 |
| Map sharpening B-factor (Å2) | −90.0 |
| Model composition | |
| Non-hydrogen atoms | 32,742 |
| Protein residues | 4126 |
| Ligands | 10 (2 for inhibitors) |
| B-factors (Å) | |
| Protein | 72.4 |
| Ligand | 64.2 |
| Rms deviation | |
| Bond length (Å) | 0.005 |
| Bond angles (o) | 0.711 |
| Ramachandran plot | |
| Favored (%) | 97.31 |
| Allowed (%) | 2.65 |
| Disallowed (%) | 0.04 |
| Validation | |
| MolProbity score | 1.03 |
| Clashscore | 1.52 |
| Poor rotamers | 0.26 |
| PDB ID | 7TZ6 |
| EMDB ID | 26,203 |