Table 2.
Studies concluding a lack of GtN conversion via PTBP1 inhibition
| Research group (reference) |
Neural region |
Targeting method | Detection method | Tracing glial origin | Disease model | Behavior benefit? |
|---|---|---|---|---|---|---|
| C.L. Zhang (Wang et al. 2021) | Striatum | AAV2/5-shRNA [same sequence as in Qian et al. (2020)] | Lineage reporter and viral reporter | Aldh1l1-CreERT2;R26R-YFP and mGfap-Cre;R26R-YFP | ND | ND |
| AAV2/5-LSL-shRNA [same sequence as in Qian et al. (2020)] | Lineage reporter and Cre-dependent viral reporter | |||||
| AAV2/2-LSL-shRNA [vector from Qian et al. (2020)] | mGfap-Cre;R26R-YFP | |||||
| AAV2/5-LSL-shRNA [vector from Qian et al. (2020)] | ||||||
| AAV2/2-LSL-shRNA [virus from Qian et al. (2020)] | ||||||
| AAV2/PHP.eB-CRISPR-CasRx [vector from Zhou et al. (2020)] | Lineage reporter and viral reporter | |||||
| S. Blackshaw (Hoang et al. 2021) | Striatum | Astrocyte-specific gene deletion | Lineage reporter | Aldh1l1-CreERT2;Sun1-GFPlox/lox;Ptbp1lox/lox | ||
| Cortex | ||||||
| SNc | ||||||
| Retina | Müller glia–specific gene deletion | Glast-CreERT;Sun1-GFPlox/lox;Ptbp1lox/lox | NMDA injury | No | ||
| B.L. Davidson (Leib et al. 2022) | Striatum | AAV2/1-miRNA [same sequence as in Xue et al. (2013)] | Lineage reporter | Aldh1l1-CreERT2;R26R-tdTomato | ND | ND |
| Cortex | ||||||
| Hippo-campus | ||||||
| M. Li (Chen et al. 2022) | Striatum | AAV2/5-shRNA [same sequence as in Qian et al. (2020)] | Lineage reporter and viral reporter | Aldh1l1-CreERT2;Rpl22lsl-HA | PD (6-OHDA) | No |
| SNc | ||||||
| ASO [same sequence as in Maimon et al. (2021)] | Lineage reporter | |||||
| B. Chen (Xie et al. 2022) | Retina | AAV2/ShH10-LSL-shRNA [vector from Qian et al. (2020)] | Lineage reporter and Cre-dependent viral reporter | Glast-CreERT;Sun1-GFPlox/lox | ND | ND |
| AAV2/PHP.eB-CRISPR-CasRx [vector from Zhou et al. (2020)] | Lineage reporter and viral reporter | |||||
| Y. Zhao (Guo et al. 2022a) | Striatum | AAV2/9-shRNA [same sequence as in Qian et al. (2020)] | Viral reporter | ND | ||
| SNc | ||||||
| Hippocampus | AD (5×FAD) | No | ||||
| AD (PS19) | ||||||
| Y. Zhao (Guo et al. 2022b) | Hippocampus | ASO [same sequence as in Qian et al. (2020)] | Neuronal density | ND | ND |
Abbreviations: 5×FAD, transgenic mouse harboring five familiar Alzheimer’s disease–linked mutations; 6-OHDA, 6-hydroxydopamine; AAV, adeno-associated virus; AD, Alzheimer’s disease; ASO, antisense oligonucleotide; GtN, glia-to-neuron; miRNA, microRNA; LSL, lox-STOP-lox; ND, not determined; NMDA, N-methyl-d-aspartate; PD, Parkinson’s disease; PS19, transgenic mouse harboring P301S tau mutation; shRNA, short hairpin RNA; SNc, substantia nigra pars compacta.