Table 1.
Identification of tyrosine phosphorylated Net1A peptides in active Src–transfected cells
| Peptide | Intensity | % | A-score | Localization | Most likely sites if not localized | Residue number |
|---|---|---|---|---|---|---|
| R.HLYQVYR.Q | 2.04e+07 | — | ||||
| R.HLpYQVYR.Q | 3.17e+05 | 1.6 | 60.66 | Yes | 370 | |
| R.HLYQVpYR.Q | 1.42e+05 | 0.7 | 64.14 | Yes | 373 | |
| R.HLpYQVpYR.Q | 5.84e+04 | 0.3 | — | Yes | 370/373 | |
| K.KGESECQYYINKLEYLDEK.Q | 3.91e+05 | — | ||||
| K.KGESECQpYYINKLEYLDEK.Q | 1.08e+05 | 27.6 | 8.94 | No | K.KGESECQpYpYINKLEYLDEK.Q | 310/311 |
| K.RQEAIYELSR.G | 5.75e+06 | — | ||||
| K.RQEAIpYELSR.G | 4.49e+05 | 7.8 | 53.2 | Yes | 126 |
Shown are the unphosphorylated and tyrosine phosphorylated (pY) Net1A peptides. Intensity refers to the mass spectrum peak area/height. Percent (%) refers to the amount of phosphorylation signal relative to total signal for that peptide. The Ambiguity score (A-score) reflects the likelihood of a particular residue being phosphorylated within a given peptide (64). Localization refers to whether the phosphorylation site was unambiguously identified. Residue number refers to the amino acid number in mouse Net1A.