TABLE 4.
SKI7 present in a high copy number cures M2 dsRNAa
| Pladmid used with ski7-1 mutant RV603 | Killer toxin secretion at the following temp (°C):
|
|
|---|---|---|
| 25 | 32 | |
| pRS316 (single-copy vector) | ++++ | +++ |
| pRS3167 (+ SKI7) | + | − |
| YEp351 (high-copy vector) | ++++ | +++ |
| YEpSKI7 (+ SKI7) | − | − |
a
The sci7-1 strain RV603 was transformed at 25°C with the single-copy plasmids pRS316 and pRS3167 and with the high-copy-number plasmids YEp351 and YEpSKI7. Transformants were streaked for single colonies at the indicated temperature, and the colonies were then tested for toxin secretion at 20°C.