TABLE 5.
Overproduction of Ski7p blocks the expression of non-poly(A) mRNAsa
| Strain | C+ A− RNA | Luciferase expression with:
|
Ratio (YEp351/YEpSKI7) | |
|---|---|---|---|---|
| YEp351 | YEpSKI7 | |||
| ski7::HIS3 3975 | UTR 24b | 12.7 | 0.40 | 31.9 |
| UTR 64b | 14.9 | 1.33 | 11.2 | |
| UTR 104b | 20.9 | 1.33 | 15.7 | |
| Wild type 4602-2B | UTR 24b | 0.16 | 0.11 | 1.5 |
| UTR 64b | 2.9 | 1.2 | 2.4 | |
| UTR 104b | 2.2 | 1.0 | 2.2 | |
| ski6-2 RV493 | UTR 24b | 10.0 | 7.9 | 1.3 |
| UTR 64b | 10.5 | 11.5 | 0.9 | |
| UTR 104b | 11.2 | 9.2 | 1.2 | |
| ski2::HIS3 3973 | UTR 24b | 19.0 | 17.3 | 1.1 |
| UTR 64b | 30.2 | 31.2 | 1.0 | |
| UTR 104b | 29.9 | 26.6 | 1.1 | |
| ski3::URA3 3974 | UTR 24b | 12.2 | 20.2 | 0.6 |
| UTR 64b | 25.9 | 25.4 | 1.0 | |
| UTR 104b | 24.6 | 33.5 | 0.7 | |
| Quadruple mutant 3963 (ski2 ski3 ski7 ski8) | UTR 24b | 15.7 | 18.4 | 0.8 |
| UTR 64b | 17.1 | 16.3 | 1.1 | |
| UTR 104b | 16.6 | 16.6 | 1.0 | |
a
The indicated C+ A− luciferase RNAs were electroporated into strains with different SKI genes and in the presence of SKI7 on a multicopy plasmid or the vector alone. Luciferase expression is reported in light units per microgram of protein. The control had less than 0.005 light unit/μg of protein. Values are averages of measurements from two independent experiments. Each measurement was made in duplicate.