TABLE 7.
Viability of xrn1/ski1 ski2 and xrn1/ski1 ski7 mutantsa
| Cross | Parents | Temp (°C) of germination | No. of xrn1 ski spores/total no. of spores |
|---|---|---|---|
| 4605 | xrn1 × ski2 | 20 | 7/48 (12/48 expected) |
| 4596 | xrn1 × ski2 | 30 | 0/70 |
| 4602 | xrn1 × ski7 | 20 | 5/24 (6/24 expected) |
| 4597 | xrn1 × ski7 | 30 | 0/76 |
a
In each cross, ski1, ski2, and ski7 were detected in the meiotic segregants by auxotrophy. Cross 4596 was D223-2A (MATa ura3 his3 trp1 xrn1::URA3) × 4588-6C (MATα ura3 his3 trp1 ski2::HIS3); crosses 4597 and 4602 were D223-2A × 4587-1A (MATα ura3 his3 trp1 leu2 ski7::HIS3); and cross 4605 was 4602-3C (MATα trp1 his3 ura3 xrn1::HIS3) × 4591-2B (MATa leu2 his3 ura3 ski2::HIS3).