Figure 7.

ConC trimer immunogenicity in guinea pigs reveals reciprocally symmetric responses

(A) Immunization scheme and groups shown for guinea pigs immunized with protein-base (blue) or glycan-base (red) ConC Env trimers.

(B) Anti-trimer ELISA responses at weeks 0, 2 and 6 are shown for each group against protein-base ConC Env (left) and glycan-base ConC Env (right).

(C) Week 6 ELISA endpoint titers using a starting dilution of 1:1000 and cross-reactivity (%) of responses are listed for each animal (left) and group comparison shown for week 6 titers against each ConC trimer (right).

(D) Electron microscopy of polyclonal epitope mapping (EMPEM) of antibodies elicited by immunization. Fabs were generate from whole plasma IgG and incubated with indicated HIV-1 Env. Negative stain EM were carried out using Env-Fab complexes purified by size exclusion chromatography, 2D averages were shown to the left of each panel, false colored in gray for trimeric particles, cyan for monomeric particles, and purple for undetermined. Representative 3D reconstructions were shown to the right with HIV-1 Env or Env fragments colored in gray, and Fabs colored by potential targeting sites. See also Tables S3 and S4.