Table 7.
Differentially abundant genera in the cecal microbiota at 2 time points among groups of animals fed a control diet or a diet supplemented with guar gum, either or not supplemented with β-mannanase at 330 g/ton feed.
| Phylum | Class | Family | Genus | CTR |
GG |
GG + E |
CTR vs. GG |
CTR vs. GG + E |
GG vs. GG + E |
|---|---|---|---|---|---|---|---|---|---|
| Mean abundance (%) | Adjusted P value | ||||||||
| Cecum 14 d | |||||||||
| Actinobacteriota | Actinobacteria | Bifidobacteriaceae | Bifidobacterium | 0 | 0.2836 | 0.0008 | <0.0001 | <0.0001 | 0.0220 |
| Actinobacteriota | Actinobacteria | Corynebacteriaceae | Corynebacterium | 0.0075 | 0.0189 | 0 | 0.5852 | NA | 0.0220 |
| Actinobacteriota | Coriobacteriia | Eggerthellaceae | Eggerthella | 0.0520 | 0.1896 | 0.0235 | 0.0069 | 0.5446 | 0.0002 |
| Firmicutes | Bacteroidia | Bacteroidaceae | Bacteroides | 1.7703 | 0.3534 | 1.0694 | 0.4688 | 0.3784 | 0.0353 |
| Bacteroidota | Bacteroidia | Rikenellaceae | Alistipes | 4.2728 | 0.6545 | 3.5875 | <0.0001 | 0.5750 | <0.0001 |
| Firmicutes | Bacilli | Bacillaceae | Bacillus | 0.7770 | 0.0806 | 0.2986 | 0.0170 | 0.2633 | 0.4078 |
| Firmicutes | Bacilli | Lactobacillaceae | HT002 | 1.0569 | 2.7934 | 0.7001 | 0.0069 | 0.5446 | 0.0002 |
| Firmicutes | Bacilli | Lactobacillaceae | Lactobacillus | 3.9770 | 11.3182 | 3.8157 | <0.0001 | 0.7878 | <0.0001 |
| Firmicutes | Bacilli | Lactobacillaceae | Limosilactobacillus | 0.0547 | 0.4844 | 0.0984 | 0.0006 | 0.7842 | 0.0220 |
| Firmicutes | Bacilli | Streptococcaceae | Streptococcus | 0.0830 | 7.7199 | 0.4476 | <0.0001 | 0.3490 | <0.0001 |
| Firmicutes | Clostridia | Defluviitaleaceae | Defluviitaleaceae UCG-011 | 0.1487 | 0.4659 | 0.0962 | 0.1933 | 0.7045 | 0.0415 |
| Firmicutes | Clostridia | [Eubacterium] coprostanoligenes group | Family_[Eubacterium] coprostanoligenes group | 1.8682 | 5.6657 | 3.4489 | 0.0170 | 0.4980 | 0.1555 |
| Firmicutes | Clostridia | Lachnospiraceae | Family_Lachnospiraceae | 2.6678 | 2.5480 | 2.2075 | 0.4118 | 0.4546 | 0.0460 |
| Firmicutes | Clostridia | Lachnospiraceae | Frisingicoccus | 0.0296 | 0.2682 | 0 | 0.1802 | 0.0093 | <0.0001 |
| Firmicutes | Clostridia | Lachnospiraceae | Marvinbryantia | 0.4971 | 2.3199 | 0.4942 | 0.0245 | 0.6922 | 0.0566 |
| Firmicutes | Clostridia | Lachnospiraceae | Roseburia | 0.0059 | 0.6579 | 0.0477 | 0.0418 | 0.4546 | 0.3246 |
| Firmicutes | Clostridia | Lachnospiraceae | Tyzzerella | 3.1932 | 2.4835 | 1.0642 | 0.9897 | 0.0093 | 0.0087 |
| Firmicutes | Clostridia | Monoglobaceae | Monoglobus | 1.3950 | 3.5698 | 1.5500 | 0.0457 | 0.9020 | 0.0307 |
| Firmicutes | Clostridia | Oscillospiraceae | Family_Oscillospiraceae | 2.5308 | 0.7232 | 0.9058 | 0.0457 | 0.0086 | 0.5377 |
| Firmicutes | Clostridia | Oscillospiraceae | Flavonifractor | 0.4751 | 0.2950 | 1.4531 | 0.8243 | 0.0043 | 0.0003 |
| Firmicutes | Clostridia | Oscillospiraceae | Intestinimonas | 5.9153 | 0.4555 | 1.7173 | <0.0001 | 0.0026 | 0.0566 |
| Firmicutes | Clostridia | Oscillospiraceae | Oscillibacter | 0.6241 | 0.3149 | 3.3616 | 0.3680 | 0.0577 | 0.0003 |
| Firmicutes | Clostridia | Ruminococcaceae | Anaerotruncus | 2.8432 | 0.0803 | 0.5591 | 0.0009 | 0.3936 | 0.0410 |
| Firmicutes | Clostridia | Ruminococcaceae | CAG-352 | 0.0016 | 0.3842 | 0.0591 | 0.0032 | 0.2258 | 0.2259 |
| Firmicutes | Clostridia | Ruminococcaceae | Caproiciproducens | 0.0241 | 0 | 0.0941 | 0.0221 | 0.2939 | 0.0007 |
| Firmicutes | Clostridia | Ruminococcaceae | DTU089 | 0.6647 | 0.1800 | 1.8231 | 0.0992 | 0.2633 | 0.0003 |
| Firmicutes | Clostridia | Ruminococcaceae | Family_Ruminococcaceae | 6.0167 | 2.0304 | 5.1728 | 0.0244 | 0.3936 | 0.2973 |
| Firmicutes | Clostridia | Ruminococcaceae | Fournierella | 0.0950 | 0.0411 | 0.0013 | 0.7692 | 0.0093 | 0.0410 |
| Firmicutes | Clostridia | Ruminococcaceae | Incertae Sedis | 4.7382 | 1.7320 | 12.2317 | 0.0244 | 0.0382 | <0.0001 |
| Firmicutes | Clostridia | Ruminococcaceae | Negativibacillus | 0.9024 | 0.2068 | 0.8357 | 0.0049 | 0.5767 | 0.0401 |
| Firmicutes | Clostridia | Ruminococcaceae | Subdoligranulum | 1.2818 | 5.5883 | 0.6227 | 0.0020 | 0.3784 | <0.0001 |
| Firmicutes | Clostridia | Peptostreptococcaceae | Family_Peptostreptococcaceae | 0.0419 | 0 | 0.0905 | <0.0001 | 0.8898 | 0.0003 |
| Proteobacteria | Gammaproteobacteria | Enterobacteriaceae | Escherichia-Shigella | 1.9624 | 6.0210 | 2.8657 | 0.0049 | 0.8246 | 0.0120 |
| Cecum 28 d | |||||||||
| Actinobacteriota | Actinobacteria | Bifidobacteriaceae | Bifidobacterium | 0 | 0.6695 | 0 | <0.0001 | 1 | <0.0001 |
| Actinobacteriota | Coriobacteriia | Eggerthellaceae | CHKCI002 | 0.1540 | 0.3567 | 0.2271 | 0.0385 | 0.6846 | 0.2387 |
| Bacteroidota | Bacteroidia | Rikenellaceae | Alistipes | 4.9440 | 2.0440 | 3.4186 | 0.0039 | 0.6444 | 0.0665 |
| Firmicutes | Bacilli | Bacillaceae | Bacillus | 1.1595 | 0.2377 | 0.8679 | 0.0417 | 0.9439 | 0.1153 |
| Firmicutes | Bacilli | Bacillaceae | Family_Bacillaceae | 0.1304 | 0 | 0.0796 | <0.0001 | 0.9687 | <0.0001 |
| Firmicutes | Bacilli | Lactobacillaceae | HT002 | 0.3678 | 1.9823 | 0.1494 | 0.0105 | 0.6651 | 0.0001 |
| Firmicutes | Bacilli | Lactobacillaceae | Lactobacillus | 0.9118 | 4.8281 | 0.8689 | 0.0003 | 1 | 0.0004 |
| Firmicutes | Bacilli | Streptococcaceae | Streptococcus | 7.9427 | 16.0682 | 6.0690 | 0.0982 | 0.9326 | 0.0186 |
| Firmicutes | Clostridia | Butyricicoccaceae | Butyricicoccus | 6.5845 | 2.0123 | 4.3069 | 0.0003 | 0.5928 | 0.0186 |
| Firmicutes | Clostridia | Butyricicoccaceae | UCG-008 | 0.0397 | 0 | 0.0046 | 0.0288 | 0.6444 | 0.2325 |
| Firmicutes | Clostridia | Butyricicoccaceae | UCG-009 | 0.1720 | 0.0328 | 0.1117 | 0.0026 | 0.7532 | 0.0253 |
| Firmicutes | Clostridia | Christensenellaceae | Christensenellaceae R-7 group | 4.2309 | 4.1057 | 2.3629 | 0.9887 | 0.1443 | 0.0349 |
| Firmicutes | Clostridia | Christensenellaceae | Family_Christensenellaceae | 0.0459 | 0.0094 | 0.0363 | 0.0480 | 1 | 0.0665 |
| Firmicutes | Clostridia | Clostridiaceae | Clostridium sensu stricto 1 | 0.0062 | 0.2236 | 0.0026 | 0.0367 | 0.7863 | 0.0023 |
| Firmicutes | Clostridia | Defluviitaleaceae | Defluviitaleaceae UCG-011 | 0.3430 | 0.4338 | 0.1992 | 0.3254 | 0.5929 | 0.0186 |
| Firmicutes | Clostridia | [Eubacterium] coprostanoligenes group | Family_[Eubacterium] coprostanoligenes group | 2.1012 | 4.6901 | 3.1611 | 0.0039 | 1 | 0.0031 |
| Firmicutes | Clostridia | Hydrogenoanaerobacterium | Family_Hydrogenoanaerobacterium | 0.0119 | 0.0020 | 0.0427 | 0.2526 | 0.6142 | 0.0167 |
| Firmicutes | Clostridia | Lachnospiraceae | [Eubacterium] hallii group | 0.1646 | 0.4483 | 0.2148 | 0.0155 | 0.9326 | 0.0599 |
| Firmicutes | Clostridia | Lachnospiraceae | [Eubacterium] ventriosum group | 0.0997 | 0.0135 | 0.0038 | 0.0340 | 0.0006 | 0.1633 |
| Firmicutes | Clostridia | Lachnospiraceae | Blautia | 1.7790 | 4.3392 | 0.8162 | 0.0059 | 0.3236 | <0.0001 |
| Firmicutes | Clostridia | Lachnospiraceae | Dorea | 0.3769 | 0.2576 | 0.0072 | 0.6096 | <0.0001 | <0.0001 |
| Firmicutes | Clostridia | Lachnospiraceae | Family_Lachnospiraceae | 1.9547 | 2.6942 | 2.3210 | 0.0043 | 0.3236 | 0.2824 |
| Firmicutes | Clostridia | Lachnospiraceae | Frisingicoccus | 0.1599 | 0.6978 | 0.0476 | 0.0518 | 0.5180 | 0.0004 |
| Firmicutes | Clostridia | Lachnospiraceae | Lachnoclostridium | 1.4047 | 0.9023 | 1.5288 | 0.2635 | 0.7863 | 0.0498 |
| Firmicutes | Clostridia | Lachnospiraceae | Lachnospira | 0.0110 | 0.3710 | 0.0267 | <0.0001 | 0.4807 | <0.0001 |
| Firmicutes | Clostridia | Lachnospiraceae | Lachnospiraceae NK4A136 group | 0.6699 | 0.8944 | 0.1580 | 0.7390 | 0.1044 | 0.0047 |
| Firmicutes | Clostridia | Lachnospiraceae | Marvinbryantia | 0.8277 | 1.5084 | 0.4420 | 0.1196 | 0.5928 | 0.0031 |
| Firmicutes | Clostridia | Lachnospiraceae | Roseburia | 0.0045 | 0.2859 | 0.0218 | 0.0043 | 0.6142 | 0.0835 |
| Firmicutes | Clostridia | Lachnospiraceae | Sellimonas | 1.0880 | 1.1354 | 0.4360 | 0.6137 | 0.0006 | <0.0001 |
| Firmicutes | Clostridia | Lachnospiraceae | Tyzzerella | 2.9827 | 1.1992 | 1.5099 | 0.0315 | 0.3886 | 0.5791 |
| Firmicutes | Clostridia | Lachnospiraceae | UC5-1-2E3 | 0.1178 | 0.4007 | 0.1730 | 0.0253 | 0.6611 | 0.1791 |
| Firmicutes | Clostridia | Monoglobaceae | Monoglobus | 0.7497 | 4.2514 | 0.5809 | <0.0001 | 0.9439 | <0.0001 |
| Firmicutes | Clostridia | Oscillospiraceae | Family_Oscillospiraceae | 2.5731 | 1.0761 | 2.9011 | 0.0182 | 0.5787 | 0.0023 |
| Firmicutes | Clostridia | Oscillospiraceae | Flavonifractor | 0.6582 | 0.2162 | 0.6112 | 0.0203 | 1 | 0.0090 |
| Firmicutes | Clostridia | Oscillospiraceae | Intestinimonas | 2.8395 | 0.5787 | 2.7148 | <0.0001 | 1 | <0.0001 |
| Firmicutes | Clostridia | Oscillospiraceae | Oscillibacter | 0.5280 | 0.3254 | 0.7632 | 0.4564 | 0.3973 | 0.0186 |
| Firmicutes | Clostridia | Oscillospiraceae | Oscillospira | 0.2566 | 0.0622 | 0.1776 | 0.0315 | 0.9439 | 0.0319 |
| Firmicutes | Clostridia | Oscillospiraceae | Pseudoflavonifractor | 0.1228 | 0.0291 | 0.1126 | 0.0658 | 1 | 0.0399 |
| Firmicutes | Clostridia | Oscillospiraceae | UCG-005 | 5.2923 | 2.4787 | 5.0788 | 0.0431 | 0.9687 | 0.0845 |
| Firmicutes | Clostridia | Peptococcaceae | Family_Peptococcaceae | 0.4498 | 0.7504 | 0.5115 | 0.0431 | 0.9326 | 0.1516 |
| Firmicutes | Clostridia | Ruminococcaceae | Anaerofilum | 0.2865 | 0.0282 | 0.0984 | 0.0272 | 0.6444 | 0.2236 |
| Firmicutes | Clostridia | Ruminococcaceae | Anaerotruncus | 0.8918 | 0.0214 | 0.4581 | <0.0001 | 0.6444 | <0.0001 |
| Firmicutes | Clostridia | Ruminococcaceae | Angelakisella | 0.0094 | 0.0013 | 0.0526 | 0.2987 | 0.6533 | 0.0348 |
| Firmicutes | Clostridia | Ruminococcaceae | CAG-352 | 0 | 0.1907 | 0 | <0.0001 | 1 | <0.0001 |
| Firmicutes | Clostridia | Ruminococcaceae | Caproiciproducens | 0.0172 | 0.0025 | 0.0372 | 0.0550 | 0.7296 | 0.0032 |
| Firmicutes | Clostridia | Ruminococcaceae | DTU089 | 0.4591 | 0.3197 | 0.6368 | 0.5802 | 0.3973 | 0.0265 |
| Firmicutes | Clostridia | Ruminococcaceae | Faecalibacterium | 12.9897 | 7.6660 | 21.6605 | 0.2828 | 0.2132 | 0.0007 |
| Firmicutes | Clostridia | Ruminococcaceae | Family_Ruminococcaceae | 5.0240 | 2.6720 | 5.4050 | 0.0059 | 0.9439 | 0.0006 |
| Firmicutes | Clostridia | Ruminococcaceae | Fournierella | 0.9912 | 0.1160 | 0.1417 | <0.0001 | 0.0006 | 0.7409 |
| Firmicutes | Clostridia | Ruminococcaceae | Incertae Sedis | 1.7218 | 1.7541 | 4.2044 | 0.6137 | 0.0006 | 0.0017 |
| Firmicutes | Clostridia | Ruminococcaceae | Paludicola | 0.4965 | 0.1426 | 0.3843 | 0.0368 | 0.9687 | 0.0665 |
| Firmicutes | Clostridia | Ruminococcaceae | Subdoligranulum | 3.0761 | 6.0777 | 1.3110 | 0.1316 | 0.3236 | 0.0004 |
| Proteobacteria | Gammaproteobacteria | Enterobacteriaceae | Escherichia-Shigella | 0.7102 | 2.5917 | 0.9393 | 0.0182 | 0.7865 | 0.0938 |
Values are the means of 12 animals (1/pen/treatment). DESeq2 analysis at 5% of significance level was used to determine statistical differences among groups.
Only bacterial families with minimum relative abundance >0.05% in at least 1 group are reported. The taxonomic classification and relative abundance of each family are shown.