Table 4.
Effects of MMDA on hematological traits at d 42 on trial (d 273 of age).
| Treatment groups | T1 | T2 | T3 | T4 | T4 | T6 | T7 | ||
|---|---|---|---|---|---|---|---|---|---|
| Hens | no | 10 | 10 | 10 | 10 | 10 | 10 | 10 | |
| Replicates | no | 10 | 10 | 10 | 10 | 10 | 10 | 10 | |
| MMDA | g/kg | 2 | 1 | 2 | 3 | ANOVA | |||
| AFB1 | mg/kg | 0.05 | 0.05 | 0.50 | 0.50 | 0.50 | |||
| T2-toxin | mg/kg | 1.5 | 1.5 | 2.0 | 2.0 | 2.0 | |||
| Body weight | |||||||||
| Body weight | g | ||||||||
| Hematological traits | |||||||||
| Erythrocytes | T/L | 2.29 ± 0.04ab | 2.13 ± 0.12a | 2.30 ± 0.08ab | 2.19 ± 0.12ab | 2.28 ± 0.04ab | 2.32 ± 0.14b | 2.23 ± 0.16ab | 0.021 |
| Leukocytes | G/L | 7.71 ± 1.89 | 7.94 ± 1.76 | 8.77 ± 5.66 | 8.97 ± 1.23 | 7.01 ± 1.03 | 6.89 ± 1.45 | 7.14 ± 1.56 | 0.610 |
| Lymphocytes | % | 42.0 ± 6.6 | 44.9 ± 6.4 | 44.1 ± 7.7 | 47.0 ± 6.1 | 37.6 ± 6.7 | 36.6 ± 6.6 | 39.0 ± 8.5 | 0.172 |
| Heterophils | % | 55.3 ± 6.8 | 51.4 ± 6.6 | 51.3 ± 10.2 | 49.6 ± 5.9 | 58.2 ± 6.9 | 58.7 ± 6.4 | 56.7 ± 8.5 | 0.122 |
| Monocytes | % | 1.4 ± 0.8 | 2.6 ± 1.1 | 3.1 ± 2.6 | 1.7 ± 1.1 | 1.6 ± 1.5 | 1.7 ± 1.1 | 1.9 ± 0.4 | 0.253 |
| Eosinophils | % | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0.4 ± 0.8 | 0.8 ± 1.1 | 0.9 ± 1.2 | 0.6 ± 0.8 | 0.119 |
| Basophils | % | 1.3 ± 0.8 | 1.1 ± 1.1 | 1.4 ± 1.1 | 1.7 ± 1.1 | 1.8 ± 1.1 | 2.1 ± 1.3 | 1.9 ± 1.2 | 0.659 |
| Hemoglobin | g/L | 72.14 ± 3.44a | 74.43 ± 3.64a | 83.14 ± 7.84bc | 73.57 ± 10.72ab | 85.00 ± 1.91c | 86.14 ± 4.49c | 84.86 ± 4.38c | <0.001 |
| Hematocrit | L/L | 0.23 ± 0.01b | 0.21 ± 0.01a | 0.22 ± 0.01ab | 0.22 ± 0.01ab | 0.22 ± 0.01ab | 0.22 ± 0.01ab | 0.21 ± 0.02ab | 0.025 |
| MCV1 | Fl | 99.41 ± 2.31c | 96.54 ± 1.15abc | 94.47 ± 2.09ab | 97.66 ± 2.22bc | 94.63 ± 2.42ab | 93.70 ± 2.10a | 94.17 ± 2.77ab | <0.001 |
| MCH2 | Pg | 31.43 ± 0.33abc | 31.32 ± 0.26ab | 38.39 ± 2.92bcd | 34.01 ± 4.03a | 39.37 ± 0.77cd | 39.66 ± 1.06cd | 40.49 ± 1.42d | <0.001 |
| MCHC3 | g/dl | 31.61 ± 0.32a | 31.33 ± 0.52a | 36.29 ± 3.07b | 29.21 ± 9.36a | 37.24 ± 0.62b | 39.66 ± 1.06b | 40.49 ± 1.42b | <0.001 |
Abbreviations: AFB1, aflatoxin B1; MCH, mean corpuscular hemoglobin, MCHC, mean corpuscular hemoglobin concentration; MCV, mean corpuscular volume; MMDA, multicomponent mycotoxin detoxifying agent.
Different superscripts in same row are significant (a/b: P ≤ 0.05).
Mean corpuscular volume;
Mean corpuscular hemoglobin;
Mean corpuscular hemoglobin concentration.