Table 2.
Primers used for a quantitative polymerase chain reaction.
| Gene1 | Accession number | Primer (5′–3′) | Product size, bp |
|---|---|---|---|
| β-ACTIN | NM_205518.2 | F: TGCGTGACATCAAGGAGAAG | 300 |
| R: TGCCAGGGTACATTGTGGTA | |||
| SREBP-1c | NM_204126.3 | F: GCCCTCTGTGCCTTTGTCTTC | 130 |
| R: ACTCAGCCATGATGCTTCTTC | |||
| FAS | NM_205155.4 | F: TGAAGGACCTTATCGCATTGC | 96 |
| R: GCATGGGAAGCATTTTGTTGT | |||
| SCD1 | NM_204890.2 | F: GTTTCCACAACTACCACCATACATT | 175 |
| R: CCATCTCCAGTCCGCATTTT | |||
| ACC | NM_205505.2 | F: GCTTCCCATTTGCCGTCCTA | 185 |
| R: GCCATTCTCACCACCTGATTACTG | |||
| ELOVL6 | NM_001031539.2 | F: GGTGGTCGGCACCTAATGAA | 169 |
| R: TCTGGTCACACACTGACTGC | |||
| CD36 | XM_038183702.1 | F: TATCGTTTCGCAGTTCCTCGTGAAG | 94 |
| R: AGTTCTGGGATATGACCTCCTCTGTAC | |||
| FATP | XM_027470622.2 | F: GCCTGATGACGTGATGTACGACTG | 113 |
| R: AGAACTTCTTGCGGATGACGATGG | |||
| FABP | NM204290 | F: GCCTGACAAAATGTGCGACC | 130 |
| R: ATTAGGCTTGGCCACACCAG | |||
| APOA1 | NM_205525.5 | F: GTGACCCTCGCTGTGCTCTT | 217 |
| R: CACTCAGCGTGTCCAGGTTGT | |||
| APOB | NM_001044633.1 | F: GGTTACTCCCACGATGGCAA | 113 |
| R: AATGCCCTTCCTTCAGGAGC | |||
| ATGL | NM_001113291.2 | F: TCCTAGGGGCCTACCACATC | 195 |
| R: CCAGGAACCTCTTTCGTGCT | |||
| PPARα | NM_001001464.1 | F: TGCTGTGGAGATCGTCCTGGTC | 166 |
| R: CTGTGACAAGTTGCCGGAGGTC | |||
| CPTL | NM_001012898.1 | F: GGAGAACCCAAGTGAAAGTAATGAA | 135 |
| R: GAAACGACATAAAGGCAGAACAGA | |||
| PGC1a | NM_001330751.2 | F: CAAAGGATGCGCTCTCGTTC | 229 |
| R: CCTCGTAGCTGTCATACCTGG | |||
| MyHC I | NM_204587.4 | F: AACGCCGCAACAACCT | 331 |
| R: TTCTTCTTCATCCGCTCC | |||
| MyHC IIa | NM_204228.4 | F: CCACCAATCCATACGACT | 216 |
| R: CTGGCTCTGCTTGCTCT | |||
| MyHC IIb | NM_001013396.2 | F: TGTGAGTCAAGGCGAGAT | 347 |
| R: CCAGAGCACCTACAGCAT | |||
| PPARγ | NM_001001460.2 | F: TGACAGCGCCAGAGATTACA | 93 |
| R: CATCCATCGCAGACAGATCCA |