TABLE 1.
High performance liquid chromatography (HPLC) determination of physalin B, L, and O contents in Physalis pubescens ethyl acetate extracts (PHY-EA).
| Compounds | Concentrations (μg/μL) | Peak area | Calibration curves | ADM of the precision (%) | Mean recovery (%) |
|---|---|---|---|---|---|
| Physalin B (1.8% ± 0.12%) | 3.00000 | 16,244.4 | y = 0.0002x + 0.0132 R 2 = 0.9997 | 0.496 | 108 |
| 1.50000 | 8,303.3 | ||||
| 0.75000 | 4,020.5 | ||||
| 0.37500 | 1,883.5 | ||||
| 0.18750 | 927.8 | ||||
| 0.09375 | 464.9 | ||||
| Physalin L (0.6% ± 0.17%) | 1.50000 | 16,043.20 | y = 0.00009 x—0.0210 R 2 = 0.99948 | 0.213 | 95 |
| 0.75000 | 8,397.90 | ||||
| 0.37500 | 4,272.40 | ||||
| 0.18750 | 2,175.00 | ||||
| 0.09375 | 1,097.40 | ||||
| Physalin O (0.3% ± 0.04%) | 0.75000 | 3,507 | y = 0.0002x + 0.0149 R 2 = 0.9997 | 0.601 | 95 |
| 0.37500 | 1,730.6 | ||||
| 0.18750 | 788.4 | ||||
| 0.09375 | 361.6 | ||||
| 0.04688 | 153 | ||||
| 0.02344 | 73.8 |
Note: the above data are from five independent experiments.