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. 2022 Oct 11;10(6):2572–2585. doi: 10.1016/j.gendis.2022.10.003

Figure 3.

Figure 3

EMC1-depletion in HRECs results in compromised Wnt signaling and cell proliferation. (A–F) Western blot and quantification analysis of the expression levels of CyclinD1, c-Myc, p-GSK3β, FZD4, and β-catenin in Ctrl and EMC1 KD HRECs. Error bars, SDs. Student's t-test (n = 3), ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. (G) RT-qPCR quantification of the relative FZD4 mRNA level in Ctrl and EMC1 KD HRECs. Error bars, SDs. Student's t-test (n = 3). ns, not significant. (H) Representative immunofluorescence images of Ctrl and EMC1 KD HRECs labeled with EdU, β-catenin, or CyclinD1 and DAPI (blue). Scale bars, 20 μm (EdU and β-catenin) and 10 μm (CyclinD1). (I–K) Quantification of the percentage of EdU + nuclei (I), and relative levels of CyclinD1 (J) and β-catenin (K) in Ctrl and EMC1 KD HRECs. Error bars, SDs. Student's t-test (n ≥ 6), ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.