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. 2022 Oct 11;10(6):2572–2585. doi: 10.1016/j.gendis.2022.10.003

Figure 6.

Figure 6

EMC1 I762V variant results in compromised Wnt signaling activity via reduced FZD4 expression. (A) Relative luciferase activity in HEK 293STF cells co-transfected with EMC1 (wild-type, wild-type/I762V, I762V variant, or vector) and pGL4-Renilla. pGL4-Renilla plasmid was transfected as an internal control. Error bars, SDs. The P-values are from multiple comparisons in one-way ANOVA with Tukey's multiple comparison test (n = 4); ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. (B–F) RT-qPCR quantification of the relative mRNA levels of EMC1, CCND1, MYC, CTGF, and DKK1 in HEK 293T cells transfected with EMC1 (wild-type, I762V variant, or vector). Error bars, SDs. The P-values are from multiple comparisons in one-way ANOVA with Dunnett's multiple comparison test (n = 3); ∗∗∗∗P < 0.0001. (G–M) Western blot and quantification analysis of the expression levels of FLAG-EMC1, FZD4, p-GSK3β, β-catenin, c-Myc, and CyclinD1 in Ctrl and EMC1 KD HRECs. Error bars, SDs. The P-values are from multiple comparisons in one-way ANOVA with Tukey's multiple comparison test (n = 3); ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. ns, not significant.