Table 1.
Molecular weight of amplification products and primer sequences for rotavirus VP4 (P) and VP7 (G) genotyping by semi-nested RT-PCR.
| Genotype | Primer | Squence (5’→3′) | Molecular weight (bp) |
|---|---|---|---|
| VP7 (G) 1st round | VP7F | ATGAATGTATTGAATATCCAC | |
| VP7 (G) 1st round | VP7R | AACATGGCACCATTTTTCC | |
| VP7(G) | VP7R | AACATGGCACCATTTTTCC | |
| G1 | aBT1 | CAAAGTACTCAATCAATATGG | 618 |
| G2 | aCT2 | CATGATATTAACCATTTACTGTG | 521 |
| G3 | G3 | ACCAACTCACACTAGAGG | 682 |
| G4 | aDT4 | CGATTCTGGTGACGAGTTG | 452 |
| G8 | aAT8 | GTCCCACCATATGTAATTCG | 754 |
| G9 | G9 | CTTATGTGCTATAATTTAC | 179 |
| VP4 (G) 1st round | VP4F | TATGCTCCAGTAAATTGG | |
| VP4 (G) 1st round | VP4R | ATTGCAATTCTTTGCATATG | |
| VP4(G) | VP4F | TATGCTCCAGTAAATTGG | |
| P[4] | 2T-1 | CTTTGTTAGAAGGTAGAGTC | 362 |
| P[6] | 3T-1 | TGTGGATTAGTTGGCTCAA | 146 |
| P[8] | 1T-1D | TCCACTGGRTAAACCTGC | 224 |
| P[9] | 4T-1 | TGACACATGCAATAGGAC | 270 |
| P[10] | 5T-1 | ATCAAAGTTAGTCGTCGG | 462 |
| P[11] | P[11] | GTAACATCCCAGACTGTG | 191 |