Figure 1.

The cell type annotation of the single-cell transcriptome atlas of mouse paravertebral muscles' developmental and aging stages. (A) Flow chart showing the experimental design for constructing a single-cell transcriptome atlas of mouse paravertebral muscle during development and aging. Cells were collected at seven timepoints: E16.5, E17.5, P0, M1, M6, M12, and M24. (B)t-SNE plot displaying the cell atlas of mouse paravertebral muscle integrating the seven timepoints. The colors represent different cell types. (C) Bubble chart indicating the expression of marker genes used for cell type annotation. The circle color represents the scale average expression of marker genes and the circle size represents the percentage of marker genes expressed. (D)t-SNE plot demonstrating integrated annotations of cells at the mouse developmental timepoints. The colors represent the timepoints. (E) Bar plot illustrating the count of up-regulated and down-regulated genes in the different cell types. Red indicates down-regulation and blue indicates up-regulation. (F) Bar plot depicting the ratios of cell types at the timepoints. The colors represent different cell types. (G)t-SNE plot presenting the expression of marker genes in the mouse paravertebral muscle cell atlas. Red indicates positive expression in a given cell.