Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 10;155(10):e202213258. doi: 10.1085/jgp.202213258

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Komondor et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Fertilization signals a PLC-mediated depolarization in X. laevis eggs. (A) Representative whole-cell recording in control conditions (diluted modified Ringer’s solution). The red arrow indicates the time of sperm addition. (B and C) Tukey box plot distributions of (B) the resting membrane potentials of eggs recorded in control conditions or in 1 μM of the PLC inhibitor U73122 and after the fertilization-evoked depolarization for control conditions, and (C) the rate of the fertilization-signaled depolarization for recordings made in control conditions. The middle line denotes the median value, the box indicates 25–75%, and the whiskers denote 10–90% (N = 28). (D) Representative whole-cell recording in 1 μM U73122. Blue arrows indicate sperm addition (N = 5). (E) PIP₂ is cleaved by PLC during the fast block to create IP₃ and diacylglycerol (DAG). Generation of IP₃ propagates the fast block to polyspermy. (F) Representative images of an undivided, two-cell stage, and polyspermic X. laevis egg.