Table 1.

Primers, product size and annealing temperatures used for identification, virulence and antimicrobial resistance genes reported in the present study

Gene	Primer sequences (5’-3’)	Product size (bp)	Annealing (°C)	References
hlyA	fw: AACAAGGATAAGCACTGTTCTGGCT	1177	60˚C	[28]
	rev: ACCATATAAGCGGTCATTCCCGTCA
phoA	fw: CGATTCTGGAAATGGCAAAAG	720 bp	60˚C	[25]
	rev: CGTGATCAGCGGTGACTATGAC
stx1	fw: ACACTGGATGATCTCAGTGG	614	58˚C	[30]
	rev: CTGAATCCCCCTCCATTATG
stx2	fw: CCATGACAACGGACAGCAGTT	779	58˚C
	rev: CCTGTCAACTGAGCAGCACTTTG
astA	fw: CCATCAACACAGTATATCCGA	110	55˚C	[29]
	rev: GGTCGCGAGTGACGGCTTTGT
stx2f	fw: AGA TTG GGC GTC ATT CAC TGG TTG	428	57˚C	[31]
	rev: TAC TTT AAT GGC CGC CCT GTC TCC
fimH	fw: TGCAGAACGGATAAGCCGTGG	508	50˚C	[32]
	rev: GCAGTCACCTGCCCTCCGGTA
eaeA	fw: ATG CTT AGT GCT GGT TTA GG	248	51˚C	[33]
	rev: GCC TTC ATC ATT TCG CTT TC
blaIMP	fw: CATGGTTTGGTGGTTCTTGT	488	53˚C	[39]
	rev: ATAATTTGGCGGACTTTGGC
blaVIM	fw: AGTGGTGAGTATCCGACA	280	53˚C
	rev: ATGAAAGTGCGTGGAGAC
blaNDM	fw: GGCGGAATGGCTCATCACGA	287	55˚C
	rev: CGCAACACAGCCTGACTTTC
blaTEM	fw: ATCAGCAATAAACCAGC	516	54˚C	[40]
	rev: CCCCGAAGAACGTTTTC
blaOXA-1	fw: ATATCTCTACTGTTGCATCTCC	619	54˚C
	rev: AAACCCTTCAAACCATCC
blaSHV	fw: AGGATTGACTGCCTTTTTG	392	54˚C
	rev: ATTTGCTGATTTCGCTCG
blaCMY	fw: GACAGCCTCTTTCTCCACA	1143	60˚C	[41]
	rev: TGGAACGAAGGCTACGTA
blaCTX-M-1	fw: GTTACAATGTGTGAGAAGCAG	1041	60˚C	[42]
	rev: CCGTTTCCGCTATTACAAAC
tetA(A)	fw: GGTTCACTCGAACGACGTCA	576	50˚C	[43]
	rev: CTGTCCGACAAGTTGCATGA
tetA(B)	fw: CCTCAGCTTCTCAACGCGTG	633	50˚C
	rev: GCACCTTGCTCATGACTCTT
sul	fw: CGGCGTGGGCTACCTGAACG		60˚C	[44]
	rev: GCCGATCGCGTGAAGTTCCG
cmlA	fw: CCGCCACGGTGTTGTTGTTATC	698	50˚C	[45]
	rev: CACCTTGCCTGCCCATCATTAG
floR	fw: TTTGGWCCGCTMTCRGAC	494	50˚C	[46]
	rev: SGAGAARAAGACGAAGAAG
mcr-1	fw: AGTCCGTTTGTTCTTGTGGC	320	58 °C	[47]
	rev: AGATCCTTGGTCTCGGCTTG
mcr-5	fw: ATGCGGTTGTCTGCATTTATC	1644	58 °C
	rev: TCATTGTGGTTGTCCTTTTCTG
chuA	fw: GAC GAA CCA ACG GTC AGG AT	279	55˚C	[27]
	rev: TGC CGC CAG TAC CAA AGA CA
yjaA	fw: TGA AGT GTC AGG AGA YGC TG	211
	rev: ATG RAG AAT GCG TTC CTC AAC
tspE4C2	fw: GAG TAA TGT CGG GGC ATT CA	152
	rev: CGC GYC AAC AAA GTA TTR CG