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. 2023 Aug 7;30:64. doi: 10.1186/s12929-023-00954-y

Table 5.

Summary of circulating SENAs associated with disease state and severity of Alzheimer’s disease in human patients

SENA Study population Sample type RNA/DNA name Findings References
miRNA

20 HC

40 AD

40 MCI

Plasma miR-483-5p

Upregulated in AD and MCI (early stage AD patients) compared to HC, decreased in AD versus MCI

Expression positively correlated with cognitive impairment

[325]

26 sMCI

19 pMCI

Whole blood miR-146a, miR-181a

Upregulated in patients with MCI who later converted to AD

Expression positively correlated with hippocampal atrophy and disconnections in critical white matter brain regions

[15]

31 HC

30 MCI

25 AD

Plasma miR-206

Upregulated in AD and MCI compared to HC

Expression positively correlated with cognitive decline in MCI patients over a 4-year longitudinal evaluation

[188]

86 HC

121 AD

Serum miR-202

Downregulated in AD patients

Expression inversely correlated with cognitive impairment

[86]

32 HC

66 AD

Serum miR-27a-3p

Downregulated in AD patients

Expression negatively correlated with disease severity

[153]
53 AD Plasma miR-342-5p Expression inversely correlated with cognitive decline over a 2-year follow-up period [65]

41 HC

116 AD

Plasma miR-21-5p, miR-126-3p

Upregulated in AD patients

Expression positively correlated with cognitive impairment

[129]

60 HC

110 AD

Serum miR-331-3p

Downregulated in AD patients

Expression inversely correlated with cognitive impairment and blood levels of pro-inflammatory cytokines

[232]

30 HC

48 AD

Serum miR-374b-5p

Downregulated in AD patients

Expression negatively correlated with cognitive impairment

[452]

19 HC

42 AD

Plasma-derived extracellular vesicles let-7 g-5p, miR126-3p, miR142-3p, miR146a-5p, mir223-3p Expression negatively correlated with disease severity [4]

45 HC

49 AD

Plasma miR-146a Expression positively correlated with cognitive impairment [249]

120 HC

20 AD

Plasma miR‐103, miR‐107 Expression inversely correlated with dementia severity [392]

23 HC

23 AD

Serum miR-34a, miR-29b, miR-181

Upregulated in AD patients

Expression positively correlated with cognitive impairment

[2]

33 HC

33 AD

Serum miR‑4722‑5p, miR‑615‑3p

Upregulated in AD patients

Expression positively correlated with cognitive impairment

[235]

106 HC

117 AD

Serum miR-128

Upregulated in AD patients

Expression positively correlated with cognitive impairment and blood levels of pro-inflammatory cytokines

[457]

18 HC

27 AD

Serum miR-501-3p

Downregulated in AD patients

Expression negatively correlated with cognitive impairment

[147]

62 HC

118 AD

Serum

CSF

miR-433

Downregulated in AD patients

Expression negatively correlated with cognitive impairment

[396]

93 HC

108 AD

Serum miR-193a-3p

Downregulated in AD patients

Expression negatively correlated with cognitive impairment

[41]

98 HC

105 AD

Serum miR-133b

Downregulated in AD patients

Expression negatively correlated with cognitive impairment

[430]

62 HC

84 AD

Serum miR-223

Downregulated in AD patients

Expression negatively correlated with cognitive impairment

[172]
lncRNA

22 HC

22 AD

Whole blood BDNF-AS

Upregulated in AD patients

Expression positively correlated with cognitive impairment

[84]

90 HC

90 AD

PBMC lncRNA-17A

Upregulated in AD patients

Expression positively correlated with cognitive impairment

[448]

32 HC

66 AD

Serum

CSF

NEAT1

Upregulated in AD patients

Expression positively correlated with disease severity

[153]

30 HC

48 AD

Serum MAGI2-AS3

Upregulated in AD patients

Expression positively correlated with cognitive impairment

[452]

120 HC

120 AD

Plasma

CSF

MALAT1

Downregulated in AD patients

Expression inversely correlated with cognitive impairment

[480]

78 HC

82 AD

Serum HOTAIR

Upregulated in AD patients

Expression positively correlated with cognitive impairment

[239]

83 HC

108 AD

PBMC GAS5

Upregulated in AD patients

Expression positively correlated with cognitive impairment and hippocampal atrophy

[49]

36 HC

45 AD

Plasma BACE1-AS

Upregulated in severely impaired AD patients

Expression positively correlated with cognitive impairment

[116]
circRNA

50 HC

50 AD

20 DLB

40 VaD

Whole blood,

Plasma

circ_0003391

Downregulated in AD, but not other types of dementia, as compared to HC

Expression negatively correlated with cognitive impairment and hippocampal atrophy

[230]

40 HC

80 AD

CSF circ_0002945 (circ-AXL), circ_0032253 (circ-GPHN)

Upregulated in AD patients

Correlated with elevated AD risk

Expression positively correlated with cognitive impairment

[217]
circ_0030777 (circ-PCCA), circ_0031258 (circ-HAUS4)

Downregulated in AD patients

Correlated with decreased AD risk

Expression inversely correlated with cognitive impairment

cfDNA

9 HC

27 AD

Plasma CNA

Increased concentration in AD patients

Concentration positively correlated with cognitive impairment

[290]

30 HC

30 AD

CSF 8-OHdG (oxidized cfDNA)

Increased concentration in AD patients

Concentration positively correlated with duration of illness

[166]

49 HC

30 spAD

16 rpAD

CSF mtDNA

Reduced copy number in spAD patients compared to HC and rpAD

Low content correlates with the earliest pathological markers of the disease, low Aβ and high p-tau, but not with the marker of neuronal damage t-tau

[303]

8-OHdG: 8-hydroxy-2-deoxyguanosine; AD: Alzheimer’s Disease; BACE1-AS: beta-secretase 1-antisense RNA; BDNF-AS: brain-derived neurotrophic factor-antisense RNA; cfDNA: cell-free DNA; circAXL: circular AXL receptor tyrosine kinase; circGPNH: circular gephyrin; circHAUS4: circular HAUS augmin-like complex subunit 4; circPCCA: circular propionyl-CoA carboxylase subunit alpha; circRNA: circular RNA; CNA: circulating nucleic acid; CSF: cerebrospinal fluid; DLB: dementia with Lewy body; GAS5: growth arrest-specific 5; HC: healthy control; HOTAIR: Hox antisense intergenic RNA; lncRNA: long non-coding RNA; MAGI2-AS3: MAGI2 antisense RNA 3; MALAT1: metastasis associated lung adenocarcinoma transcript 1; MCI: mild cognitive impairment; miRNA: microRNA; mtDNA: mitochondrial DNA; NEAT1: nuclear paraspeckle assembly transcript 1; PBMC: peripheral blood mononuclear cells; pMCI: progressor MCI (subjects who progress to AD); rpAD: rapid progressive AD; SENA: self-extracellular nucleic acid; sMCI: stable MCI (subjects which remain cognitively stable over time); spAD: slow progressive AD; VaD: vascular dementia