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Instrumental parameters for the chromatographic separation using the Agilent 1260 HPLC
| Autosampler temperature [°C] | 37 |
| Injection volume [µl] | 5 |
| Column | Acquity UPLC Protein BEH SEC 4.8 × 300 mm, 1.7 µm, 200 Å |
| Eluent (isocratic) | 50 mM CH3COONH4 pH 6.8 |
| Flow rate [µl min−1] | 400 |
| Measurement time [min] | 12 |
